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flow cytometry fixation buffer cat. pf00016  (Proteintech)


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    Structured Review

    Proteintech flow cytometry fixation buffer cat. pf00016
    The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
    Flow Cytometry Fixation Buffer Cat. Pf00016, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+fixation+buffer+cat%2E+pf00016/flow+cytometry+fixation+buffer+cat++pf00016/pmc12005212-48-0-16
    Average 90 stars, based on 1 article reviews
    flow cytometry fixation buffer cat. pf00016 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway"

    Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S509734

    The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
    Figure Legend Snippet: The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

    Techniques Used: Flow Cytometry, Expressing, Co-Immunoprecipitation Assay

    Related Articles

    Flow Cytometry:

    Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway
    Article Snippet: PE anti-mouse CD206 (Cat 141705) was obtained from BioLegend (San Diego, CA, USA). .. Flow cytometry fixation buffer (Cat. PF00016), and Flow Cytometry Perm Buffer (Cat. PF00017) were purchased from Proteintech (Hubei, China). .. Lipopolysaccharide (LPS) (CAS 297–473-0) and the CCK-8 Kit (BS350B) were purchased from Biosharp (Guangdong, China).



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    flow cytometry fixation buffer cat. pf00016  (Proteintech)
    90
    Proteintech flow cytometry fixation buffer cat. pf00016
    The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
    Flow Cytometry Fixation Buffer Cat. Pf00016, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+fixation+buffer+cat%2E+pf00016/flow+cytometry+fixation+buffer+cat++pf00016/pmc12005212-48-0-16
    Average 90 stars, based on 1 article reviews
    flow cytometry fixation buffer cat. pf00016 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

    Journal: Journal of Inflammation Research

    Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway

    doi: 10.2147/JIR.S509734

    Figure Lengend Snippet: The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

    Article Snippet: Flow cytometry fixation buffer (Cat. PF00016), and Flow Cytometry Perm Buffer (Cat. PF00017) were purchased from Proteintech (Hubei, China).

    Techniques: Flow Cytometry, Expressing, Co-Immunoprecipitation Assay